PRACTICAL4 Introduction The purpose of this experiment is to demonstrate viral specificity. In this applicatory viral specificity is employ as a barb to encounter the enigmatical bacteria. Known bacterio bacteriophages undersurface be used to date unknown bacteriophages by observing whether the bacteria are lysed or non. DISCUSSION There strains that leave behind be affected by the bacteriophages and there are those that result not be affected. Those that lack the viral sense organ pass on show no reduction of the growth in the circulated regulate. The keep down of reduction of growth in sensitive strains will estimate on the viral specificity of the bacteriophage particles in the phage taproom and on whether the virus is virulent or temperate. irate phages will show little or a big geographical regularise of inhibition where the phage drops has been inoculated. Many bacteriophages are temperate and bath establish a latent infection of their host termed lysog eny. In this state, the viral genome is known as a prophage, and the host is set up to be lysogenized or a lysogen. Summary of procedure both phages of E .coli are inoculated in 2 separate streamlet metros containing sterile PBS, and the test supply are named one and two respectively. Then intravenous feeding plates inoculated with four different unknown bacteria are named 1-4 respectively. Then individually plate is carve up into two halves using a soft touch pen.
On each half, a band is drawn and where the circle is drawn, a drop of PBS phage solution is placed. A circle where a drop is placed is where a viable partition of inhibition will occu! r depending on the metier of the phage solution. Results obtained Bacteria Test pipe 1         Test tube 2 1         No clear zone         No clear zone 2         No clear zone         No clear zone 3         Clear zone         No... If you want to get a full essay, order it on our website:
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