beet lab :: essays research papers

MATERIAL AND METHODSStability of cellphone membrane using pHFor the science laboratory experiment for analyzeing the stability of beet cell membranes using pH, many materials were employ as follows. Obtaining a beet we puncher out cores, using a phellem borer. subsequently washing the cores we swan apiece one inside a separate test tube, and added a different pH ancestor in each one. After 3 minutes in these exposure solutions, we took the beet out with a dissecting harass. wherefore transferred each beet to a separate test tube containing deionized water. After 20 minutes in these diffusion solutions, we took the beets out with a dissecting needle and discard it. We then stirred each solution in the test tube with a stirring rod, and transferred it to a cuvette. A spectrophotometer was then calibrated, and used to measure the absorbance of each exposure solution, and diffusion solution. Membrane DamageFor the lab experiment for Membrane Damage, we tested the extract pi gment and diluted it. When the pH solutions are added, this will cause it to be in a come out of absorbance. We used materials as follows. Obtaining a beet we proceeded to cut small individual(a) cubes. We then rinse each cube to remove any discredited pigments with deionized water. Using a blender, we blend the beets with 15 mL of pH 7 DI water. After blending we used cheesecloth to separate the liquid from the solids for easier separator process. consequently we put the liquid beet into a centrifuge tube and centrifuge it for 5 minutes at 2500 rpm. We then remove the supernatant into a beaker, and discarded the sediment. Using a 14 ratio mixed bag of the supernatant and deionized water, we made a bourgeon solution. We then tested the stock solutions absorbance with a spectrophotometer, and place 1 mL of the solution into separate test tubes. Next we added an additional 4 mL of pH solutions in the 2-11 range into each test tube. After mixing, we tested the absorbance for each solution using a spectrophotometer. Mixing the Diffusion and Exposure firmnessFor the lab experiment of mixing the diffusion and exposure solution, we are dismissal to test if the pigment is released in the exposure solution. We used materials as follows Obtaining a beet, we punched out 2 cm long beets using a cork borer. Then we wash the beets in running water, after that we prepared 10 test tubes each containing different pH solution.